Table 1. The sequences of polymerase chain reaction (PCR) primers used in this study.

Peptide/ fragment	Amino acid co-ordinates	Base pair co-ordinates	Forward primer1	Reverse primer1	Product size (bp)
p1	1–12	1–36	AGGGTACCAACATCTTTGAG	TGTCTAGAGGGGCGGAGTGG	58
p306	306–320	916–960	AGGGTACCGATGGTCACTACCAA	TGTCTAGACTGGGCATCCACACA	79
p1579	1579–1591	4735–4773	AGGGTACCGACTCCCCGCTGGTG	TGTCTAGAGAAGCTGCAGGCCTC	72
p1672	1672–1711	5014–5133	AGGGTACCCAGAAGAGCTTCGAA	TGTCTAGAACAGCAGGAATCATT	154
p1826	1826–1836	5476–5508	AGGGTACCGACTTTCCAGGAGAT	TGTCTAGAGGTAATGTCCACAGG	67
p2102	2102–2116	6304–6348	AGGGTACCAGTAACTTCTCCATG	TGTCTAGAAAGGCAGTCCTGGTG	79
p2340	2340–2359	7018–7077	AGGGTACCCTGCTGGACCAAGTG	TGTCTAGATGTCACACGCTGAGG	94
p2494	2494–2510	7483–7533	CGGGTACCATGGGGCTTATCAATAG	CGTCTAGATCAGCCTTGGCTCTCTT	73
p2549	2549–2560	7645–7680	AGGGTACCTTGGAGCACTCCACA	TGTCTAGAGGCATTCTCCAGTGC	70
p2596	2596–2608	7786–7824	AGGGTACCCCCGAAAGCTATGGC	TGTCTAGAAAAAGCATATTGAAC	73
p2695	2695–2713	8083–8136	CGGGTACCATGTGCTCCTTCTGGT	CGTCTAGATCATGCATCCTTGGCTC	82
p2730	2730–2743	8188–8229	AGGGTACCGTTGGACCTGGATTA	TGTCTAGATTTGCTGTAGCTCTT	82
A	306–520	916–1560	AGGGTACCAACATCTTTGAG	TGTCTAGACTTCTCAGACACACG	645
B	516–745	1546–2235	ATGGTACCCGTGTGTCTGAGAAG	TGTCTAGAAGAGGCACTGCACTGAGG	690
C	741–980	2221–2940	AGGGTACCCCTCAGTGCAGTGCC	TGTCTAGACTGAGCAGCCAAGCG	720
D	976–1225	2926–3675	AGGGTACCCGCTTGGCTGCTCAG	TGTCTAGACTGCTGAACAGTCGT	750
E	1221–1435	3661–4305	AGGGTACCACGACTGTTCAGCAG	TGTCTAGAAGCATCCTGTCTGGT	645
F	1436–1711	4306–5133	AGGGTACCCTGGGCTGTGTGAAA	TGTCTAGAACAGCAGGAATCAT	828
β-actin	–	–	GCTCTTTTCCAGCCTTCCTT	CTTCTGCATCCTGTCAGCAA	177

¹All primers are given in the 5′ to 3′ direction; the underlined sequence is the KpnI site in the forward primer and the XbaI site in the reverse primer.