Figure 4.
PPT16 antigen is expressed at the surface of γδ-T cells. PBL (lanes 1–3), PPT16– cells (lanes 4–6) and CD4– CD8– cells (lanes 7, 9) were 125I iodinated, lysed in 1% digitonin buffer and precipitated with anti-CD3-ε (lanes 1, 4, 7), PPT16 (lane 2, 5, 8), or normal mouse serum (lanes 3, 6, 9). The 10% SDS–PAGE gel was run under nonreducing conditions.