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(a) Expression of fusion proteins. COS-7 cells were transfected with indicated plasmids for 48 hr with LipofectAmine plus reagent. The expression of GFP was revealed under a fluorescent microscope.(b)Western blot. Transfected COS-7 cells were analysed by Western blot using anti-polyhistidine mAb.(c)GFP-CD40LT binding to B cells. Purified mouse B cells were incubated with the supernatant of COS-7 cells transfected with pGFP-CD40LT or mock transfection.
