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. 2005 Jun;115(2):215–222. doi: 10.1111/j.1365-2567.2005.02141.x

Figure 3.

Figure 3

(a) Synergistic induction of GFP-specific antibodies by pGFP-CD40LT. Mice (5–7 weeks old, five mice/group) were immunized s.c. with indicated plasmid(s) (50 µg/plasmid/mouse) at day 0 and day 14. Blood was collected at day 21. Anti-GFP antibody titre was analysed by ELISA using biotin-labelled anti-mouse total IgG secondary antibody, as described in Methods. (b) pGFP-CD40LT induced high level anti-GFP antibodies without booster. Mice were immunized with indicated plasmid with or without booster, as indicated. Blood was collected at day 21 and analysed for anti-GFP antibodies. These data represent three separate experiments.