Table 2. Per cent TCR-αβ+ CD8+ tetramer and per cent bulk TCR-αβ+ CD8+ (tetramer minus) in parentheses expressing each marker among six volunteers from the FluM1 group12.
| Subject ID | ||||||
|---|---|---|---|---|---|---|
| Marker | 01 | 02 | 03 | 04 | 05 | 06 |
| CD27 | 58 (71) | 91 (92) | 97 (85) | 99 (81) | 99 (57) | 100 (72) |
| CD28 | 24 (48) | 74 (82) | 93 (73) | 86 (76) | 96 (58) | 98 (67) |
| CD45RA | 6 (25) | 7 (54) | 16 (38) | 1 (28) | 7 (32) | 3 (41) |
| CD62L | 35 (45) | 38 (47) | 40 (46) | 41 (63) | 28 (48) | 70 (60) |
| CD94 | 63 (14) | 60 (2) | 32 (9) | 82 (15) | 45 (7) | 11 (17) |
| HLA-DR | 20 (24) | 19 (12) | 12 (26) | 15 (28) | 6 (34) | 1 (14) |
| Granzyme A | 79 (70) | 73 (33) | 52 (55) | 98 (56) | 20 (65) | 53 (38) |
| Perforin | 68 (27) | 27 (5) | 5 (14) | 27 (20) | 5 (29) | 4 (33) |
| CXCR3 | 32 (24) | 62 (36) | 83 (53) | 99 (85) | 85 (74) | 90 (57) |
| CXCR4 | 19 (18) | 39 (64) | 23 (32) | 52 (41) | 97 (88) | 59 (52) |
| CXCR5 | 6 (4) | 6 (2) | 9 (4) | 1 (2) | 12 (1) | 7 (1) |
| CCR5 | 57 (34) | 20 (13) | 42 (30) | 91 (41) | 29 (37) | 44 (37) |
| CCR6 | 18 (7) | 6 (13) | 4 (6) | 2 (10) | 15 (10) | 3 (6) |
| CCR7 | 12 (22) | 27 (64) | 16 (35) | 2 (40) | 21 (17) | 33 (38) |
1
Per cent marker expression is derived from the number of marker-positive tetramer + events (upper right quadrant of plots in Figs 1 and 2) or tetramer– (bulk CD8+ T cells) events (bottom right quadrant) divided by number of total tetramer+ or tetramer– (bulk CD8+ T cells) events (Upper or bottom right and left quadrants).
2
Markers shown in bold or underlined across the donor indicate that the mean per cent marker expression of the tetramer + CD8+ T cell is significantly lower (bold) or higher (underline) than that of the tetramer– CD8+ T cell (bulk CD8+ T cells) with P < 0·05, determined by the paired Student's t-test. See the graphical representation of these values in Fig. 3.