Table 3. Per cent TCR-αβ+ CD8+ tetramer+ and per cent bulk TCR-αβ+ CD8+ (tetramer–) in parentheses expressing each marker among five volunteers from the BMLF1 group1, 2.
| Subject ID | ||||
|---|---|---|---|---|
| Marker | 01 | 07 | 08 | 09 |
| CD27 | 81 (73) | 83 (88) | 64 (93) | 95 (78) |
| CD28 | 26 (49) | 24 (81) | 35 (77) | 92 (70) |
| CD45RA | 13 (28) | 20 (63) | 90 (84) | 2 (38) |
| CD62L | 33 (47) | 16 (59) | 7 (44) | 41 (67) |
| CD94 | 26 (19) | 26 (6) | 25 (3) | 44 (11) |
| HLA-DR | 34 (18) | 27 (7) | 32 (9) | 19 (8) |
| Granzyme A | 93 (74) | 95 (51) | 91 (31) | 91 (48) |
| Perforin | 42 (33) | 26 (8) | 16 (8) | 24 (23) |
| CXCR3 | 25 (32) | 71 (59) | 55 (63) | 81 (61) |
| CXCR4 | 6 (20) | 13 (56) | 35 (67) | 2 (16) |
| CXCR5 | 2 (13) | 3 (0) | 8 (3) | 1 (3) |
| CCR5 | 40 (33) | 43 (22) | 29 (7) | 89 (27) |
| CCR6 | 2 (8) | 6 (15) | 1 (6) | 4 (5) |
| CCR7 | 2 (18) | 5 (68) | 7 (59) | 18 (53) |
1
Per cent marker expression is derived from the number of marker-positive tetramer + events (upper right quadrant of plots in Figs 1 and 2) or tetramer– (bulk CD8+ T cells) events (bottom right quadrant) divided by number of total tetramer+ or tetramer– (bulk CD8+ T cells) events (upper or bottom right and left quadrants).
2
Markers shown in bold or underlined across the donor indicate that the mean per cent marker expression of the tetramer + CD8+ T cell is significantly lower (bold) or higher (underline) than that of the tetramer– CD8+ T cell (bulk CD8+ T cells) with P < 0·05, determined by the paired Student's t-test. See the graphical representation of these values in Fig. 3