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. 2005 Jul;115(3):366–374. doi: 10.1111/j.1365-2567.2005.02160.x

Figure 1.

Figure 1

Effects of tyrphostin and genistein AG126 on LTA-mediated nitrite release and iNOS expression in RAW 264.7 macrophages. In (a), cells were pretreated with various concentrations of tyrphostin AG126 or genistein for 30 min followed by LTA (10 µg/ml) treatment for 24 hr. The supernatant was collected for nitrite measurement. Results are expressed as the mean ± SEM of three independent experiments performed in triplicate. *P < 0·05 as compared with the LTA-treated group. In (b), cells were pretreated with tyrphostin AG126 (20 µm) or genistein (30 µm) for 30 min followed by LTA (10 µg/ml) treatment for 24 hr, and then immunodetected using specific antibodies against iNOS or α-tubulin as described in ‘Materials and methods’. Typical traces represent three experiments with similar results. Equal loading in each lane is demonstrated by similar intensities of α-tubulin. Tyrp, tyrphostin AG126; Geni, genistein.