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. 2005 Jul;115(3):366–374. doi: 10.1111/j.1365-2567.2005.02160.x

Figure 2.

Figure 2

Effects of wortmannin and LY 294002 on LTA-mediated nitrite release and iNOS expression in RAW 264.7 macrophages. In (a), cells were pretreated with various concentrations of wortmannin or LY 294002 for 30 min followed by 10 µg/ml LTA treatment for 24 hr, and then the media were collected for nitrite measurement. Results are expressed as the mean ± SEM of three independent experiments performed in triplicate. *P < 0·05 as compared with the LTA-treated group. In (b), cells were pretreated with various concentrations of wortmannin or LY 294002 for 30 min followed by 10 µg/ml LTA treatment for 24 hr, and then immunodetected using specific antibodies against iNOS or α-tubulin as described in ‘Materials and methods’. Typical traces represent three experiments with similar results. Equal loading in each lane is demonstrated by similar intensities of α-tubulin. Wort, wortmannin; LY, LY 294002.