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. 2005 Jul;115(3):358–365. doi: 10.1111/j.1365-2567.2005.02174.x

Figure 1.

Figure 1

Expression of RGS mRNA in rat leucocytes. (a) RT—PCR analysis of RGS transcripts performed on total RNA isolated from freshly isolated rat NK cells or rat NK cells cultured for 7 days in the presence of IL-2. Products were visualized by agarose gel electrophoresis, and length of products determined with a 100 bp ladder (Roche, Indianapolis, IN). CD45 was included as a control for equal amounts of cDNA. (b) RT—PCR analysis of RGS in freshly isolated rat haematopoietic cells from PVG rats and cell lines as indicated. R2 is a macrophage cell line, RBL-2H3 is a basophilic leukaemia, RNK-16 and A181 are NK cell lines derived from F344 rats. Negative control represents water subjected to first-strand cDNA synthesis plus PCR. The experiments were repeated twice to ensure reproducibility.