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. 2005 Aug;115(4):506–515. doi: 10.1111/j.1365-2567.2005.02185.x

Figure 2.

Figure 2

Increased IL-8 mRNA expression in INT-407 and Caco-2 cells by V. vulnificus infection. Human intestinal epithelial INT-407 cells and Caco-2 cells were, respectively, infected with V. vulnificus at 10 MOI for 5–60 min (a) or various MOIs for 60 min (b). Cellular RNA at each point was extracted and IL-8 mRNA expression was determined by RT-PCR. The RT-PCR products were analysed in 2·0% agarose gels. (c) INT-407 cells were treated with 10 μg/ml actinomycin D for 1 hr, 1·5 hr and 2 hr, followed by V. vulnificus infection for 1 hr at 10 MOI. The mRNA levels of IL-8 and GAPDH as a control were detected by Northern blot analysis. The data are representative of two independent experiments.