RNase protection assay with the set of coagulation/fibrinolytic genes. (a) Typical eight-band protection pattern resulting from the analysis of 5 μg of mouse-skin total RNA. (b) Kidney RNA prepared from various knockout (KO) mice (PAI1-, UPA-, or PAR1-deficient mice) and from wild-type (WT) mice, analyzed using RPA. (c) Kidney RNA prepared from lipopolysaccharide (LPS)-treated or untreated mice, analyzed using RPA. (d) RNA from murine hypoxic or normal endothelial cells, analyzed using RPA. In all these RPA experiments, 5 μg of total RNA/lane was analyzed.