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Changes in human leucocyte antigen (HLA)-DR, CD86 and CD80 expression during maturation of monocyte-derived dendritic cells (MoDCs) and MUTZ-3 DCs, as assessed by flow cytometry. Immature MoDCs (a, e) were stimulated with a proinflammatory cocktail for 48 hr (b, f). Similarly, differentiated immature MUTZ-3 DCs (c, g) were allowed to mature for 48 hr after stimulation (d, h). FITC, fluorescein isothiocyanate; PE, phycoerythrin.
