Table 1. Dendritic cell markers expressed by monocyte-derived dendritic cells (MoDCs) and MUTZ-3 dendritic cells (MUTZ-3-DCs) in their immature (0 hr) and mature (48 hr) states.
MoDCs | MUTZ-3-DCs | ||||
---|---|---|---|---|---|
GenBank no. | Description | 0 hr | 48 hr | 0 hr | 48 hr |
NM_001561 | 4-1BB | – | ++ | + | ++ |
NM_003811 | 4-1BB-L | +– | ++ | + | ++ |
NM_006401 | APRIL | +++ | +++ | +++ | ++ |
NM_004925 | AQP3 | ++ | – | ++ | – |
NM_001295 | CCR1 | ++ | – | + | + |
NM_000647 | CCR2 | – | – | ++ | + |
NM_000579 | CCR5 | ++ | – | +– | – |
NM_001838 | CCR7 | + | +++ | +– | +++ |
NM_000632 | CD11b | ++ | – | ++ | ++ |
M81695 | CD11c | ++ | – | – | +– |
NM_001150 | CD13 | ++ | ++ | ++ | +++ |
M28825 | CD1a | +++ | +– | ++ | +++ |
NM_006110 | CD2 | ++ | ++ | ++ | ++ |
NM_002002 | CD23 | ++ | + | ++ | + |
NM_000417 | CD25 | – | ++ | – | – |
NM_021642 | CD32 | ++ | – | ++ | ++ |
NM_001772 | CD33 | ++ | – | ++ | ++ |
M81104 | CD34 | – | – | ++ | + |
NM_001250 | CD40 | ++ | ++ | ++ | ++ |
BC004372 | CD44 | ++ | ++ | +++ | +++ |
NM_001251 | CD68 | + | – | + | +– |
NM_005191 | CD80 | ++ | ++ | ++ | ++ |
NM_004233 | CD83 | ++ | +++ | ++ | +++ |
BG236280 | CD86 | ++ | ++ | ++ | ++ |
AF348491 | CXCR4 | +– | +++ | ++ | ++ |
NM_014398 | DC-LAMP | ++ | +++ | +– | +++ |
AF290886 | DC-SIGN | +++ | – | ++ | ++ |
NM_002349 | DEC-205 | ++ | +++ | + | ++ |
NM_006140 | GM-CSFR | ++ | +++ | ++ | ++ |
NM_000201 | ICAM-1 | ++ | +++ | ++ | +++ |
NM_002187 | IL-12Bp40 | + | ++ | + | ++ |
NM_000877 | IL-1R1 | ++ | +++ | +– | ++ |
NM_004633 | IL-1R2 | ++ | ++ | ++ | +++ |
NM_000418 | IL-4RA | ++ | ++ | ++ | ++ |
NM_003326 | OX40L | – | ++ | – | ++ |
NM_000958 | PGER | ++ | ++ | ++ | ++ |
NM_003839 | RANK | – | ++ | – | + |
NM_003264 | TLR2 | + | – | ++ | ++ |
NM_003266 | TLR4 | ++ | – | +– | – |
NM_001065 | TNFR1 | ++ | ++ | ++ | ++ |
NM_001066 | TNFR2 | ++ | ++ | + | + |
AA573862 | HLA-A | +++ | +++ | +++ | +++ |
L42024 | HLA-B | +++ | +++ | +++ | +++ |
U655851 | HLA-DR | +++ | +++ | +++ | +++ |
GM-CSFR, granulocyte–macrophage colony-stimulating factor; ICAM, intercellular adhesion molecule; IL, interleukin; TLR, Toll-like receptor; TNF, tumour necrosis factor; HLA, human leucocyte antigen.
Fluorescence signal intensity values are indicated as follows: not expressed (–), fluorescence signal intensity value <100 (+), 100–1000 (++) and >(+++). Genes expressed in one array out of two (+−).