Table 1.

Culture with GM-CSF does not alter the expression of chemotactic receptors

	3 hr medium	3 hr GM-CSF	6 hr medium	3 hr medium+ 3 hr GM-CSF
CXCR	367 ± 28	344 ± 63	384 ± 85	434 ± 111
CXCR2	69 ± 26	52 ± 47	90 ± 38	44 ± 37
FPR	17 ± 3	30 ± 19	28 ± 13	30 ± 14
MHC I	114 ± 45	136 ± 61	117 ± 46	111 ± 47
CD45	438 ± 147	324 ± 188	390 ± 174	387 ± 221

Neutrophils of three donors were treated for 3 hr with medium or GM-CSF (4 ng/ml) and then stained for surface expression of chemotactic receptors CXCR1, CXCR2 and formyl peptide receptor (FPR), and of Class I MHC and CD45 as controls. Separate aliquots of cells were cultured in medium for 6 hr, or in GM-CSF (4 ng/ml) for 3 hr followed by medium for 3 hr, before staining. Results are expressed as mean of MFI of cells from the three donors followed by standard error. Staining used 1 μg of antibody per 10⁶ cells and all values are corrected for isotype control staining.