Figure 1.

Differential chemotactic responses toward CCL19 and CXCL12. The chemotactic responsiveness of mature DC toward the CCR7 ligand CCL19 and the CXCR4 ligand CXCL12 was tested by performing migration assays using 96-well chemotaxis chambers with 5-μm pore polycarbonate filters. Mature monocyte-derived DC (10⁵ cells) suspended in medium were loaded into the upper well and RPMI-1640 containing the probed chemokines at a concentration of 200 ng/ml was placed in the lower wells, while each condition was set up in triplicate. DC that had migrated through the filter to the lower wells were collected after 90-min incubation and counted completely by flow cytometry. One representative experiment of three independent experiments with similar results is shown. Data represent the mean + SD of triplicates.