Table 1.
Dermal cellular infiltrate before and after intradermal injection of polyclonal rabbit IgG anti-IgE or normal rabbit IgG
| Normal rabbit IgG (n=3) | Anti-IgE, no treatment (n=5) | Anti-IgE, prednisolone-treated (n=5) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Baseline | 6hr | 24hr | 48hr | Baseline | 6hr | 24hr | 48hr | Baseline | 6hr | 24hr | 48hr | |
| Total nucleated cells | ||||||||||||
| Mean | 309.3 | 474.3 | 362.0 | 366 | 212.4 | 471** | 815.6*** | 527** | 193.8 | 276.4 | 471* | 273 |
| (SE) | (44.4) | (65.2) | (60.8) | (39.7) | (30.6) | (55.3) | (221.4) | (82.5) | (23.5) | (46.3) | (186.7) | (49.8) |
| Median | 345 | 535 | 347 | 351 | 187 | 514 | 774 | 503 | 223 | 234 | 215 | 314 |
| Fold change | 1.6 | 1.2 | 1.2 | 2.2 | 2.6 | 2.7 | 1.6 | 2.0 | 1.4 | |||
| Intact mast cells | ||||||||||||
| Mean | 23.2 | 18.3 | 15.3 | 12.7 | 12.4 | 3.4** | 2.4*** | 7.2 | 13 | 3.8** | 2.** | 10 |
| (SE) | (2.9) | (9.4) | (5.4) | (5.2) | (3.5) | (1.9) | (1.7) | (2.3) | (3.4) | (1.2) | (1.2) | (2.8) |
| Median | 24 | 10 | 11 | 8 | 10 | 1 | 1 | 8 | 14 | 3 | 2 | 7 |
| Fold change | −1.1 | −1.8† | −2.3 | −8.6 | −10.6 | −2.3 | −4.1 | −9.0 | −0.7 | |||
| Eosinophils | ||||||||||||
| Mean | 2.0 | 19.7 | 8.7 | 5.7 | 0.2 | 68** | 38.8* | 41.8* | 0 | 0 | 1 | 0 |
| (SE) | (1.5) | (5.5) | (5.4) | (4.3) | (0.2) | (15.7) | (11.6) | (12.8) | (0) | (0) | (1.0) | (0) |
| Median | 1 | 23 | 6 | 3 | 0 | 55 | 42 | 47 | 0 | 0 | 0 | 0 |
| Fold change | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | |||
| Neutrophils | ||||||||||||
| Mean | 8.3 | 95.7*** | 55.3** | 22 | 7 | 62.2*** | 114*** | 65.4*** | 12.2 | 31.4 | 75.6*** | 37.8* |
| (SE) | (2.3) | (26.7) | (20.5) | (4.6) | (1.8) | (10.8) | (32.0) | (21.9) | (3.6) | (11.1) | (31.0) | (12.1) |
| Median | 10 | 101 | 40 | 19 | 6 | 57 | 84 | 43 | 11 | 25 | 30 | 52 |
| Fold change | 16.4 | 12.2 | 2.9 | 12.9 | 21.9 | 17.6 | 2.2 | 7.3 | 3.1 | |||
| CD1c+ | ||||||||||||
| Mean | 82.7 | 45 | 117 | 73.7 | 23 | 53.8** | 150.4*** | 107.2*** | 23 | 47* | 81.8* | 59.6** |
| (SE) | (9.8) | (5.2) | (17.3) | (18.2) | (5.1) | (11.8) | (11.7) | (19.2) | (5.1) | (8.7) | (35.6) | (18.6) |
| Median | 76 | 45 | 117 | 76 | 24 | 40 | 158 | 95 | 19 | 45 | 61 | 45 |
| Fold change | −1.9† | 1.6††† | −0.6†† | 2.8 | 8.4 | 6.1 | 2.6 | 2.8†† | 2.5† | |||
| CD3+ | ||||||||||||
| Mean | 35.7 | 25 | 88.7 | 50 | 12.6 | 19 | 73.6*** | 52.2*** | 14 | 11.6 | 32 | 23.4 |
| (SE) | (6.4) | (9.9) | (14.3) | (18.9) | (3.3) | (3.3) | (16.4) | (10.8) | (2.7) | (2.6) | (13.4) | (10.4) |
| Median | 40 | 20 | 96 | 38 | 11 | 20 | 99 | 49 | 13 | 11 | 36 | 9 |
| Fold change | −1.4 | 3.3† | 0.7† | 1.7 | 7.8 | 4.9 | −0.1 | 0.4†† | 0.1† | |||
***
P<0.001
**
P<0.01
*
P<0.05, relative to baseline cell numbers.
†††
P<0.001
††
P<0.01
†
P<0.05, decreased fold change compared to that seen in time-matched, anti-IgE-injected skin in untreated dogs.