Table 2.
Changes in cytokine and chemokine mRNA expression after intradermal injection of polyclonal rabbit IgG anti-IgE or normal rabbit IgG
| Messenger RNA expression (in units relative to Ubiquitin) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Normal rabbit IgG (n=3) | Anti-IgE, no treatment (n=5) | Anti-IgE, prednisolone-treated (n=5) | ||||||||||
| Baseline | 6hr | 24hr | 48hr | Baseline | 6hr | 24hr | 48hr | Baseline | 6hr | 24hr | 48hr | |
| CCL2 (MCP-1) | ||||||||||||
| Mean | 3.4 | 90.7** | 26.7 | 9.1 | 2.9 | 109.0*** | 4.8 | 13.5 | 2.2 | 15.2** | 5.0 | 7.2 |
| (SE) | (0.8) | (10.2) | (7.2) | (1.5) | (1.1) | (67.3) | (1.3) | (6.2) | (1.1) | (1.2) | (2.0) | (3.7) |
| Median | 3.1 | 90.4 | 20.6 | 7.8 | 4.1 | 41.6 | 5.7 | 12.6 | 1.8 | 16.4 | 3.9 | 3.8 |
| Fold change | 28.2 | 7.8 | 3.0 | 104.7 | 12.0 | 13.9 | 41.6 | 11.3 | 9.5 | |||
| CCL5 (RANTES) | ||||||||||||
| Mean | 1.1 | 3.9 | 3.7 | 3.0 | 1.2 | 6.3** | 1.6 | 3.8 | 0.9 | 1.7 | 0.8 | 1.6 |
| (SE) | (0.1) | (1.2) | (0.6) | (1.0) | (1.0) | (2.4) | (1.2) | (2.5) | (0.6) | (0.8) | (0.4) | (0.6) |
| Median | 1.1 | 3.1 | 3.8 | 2.4 | 0.2 | 4.3 | 0.7 | 0.9 | 0.4 | 0.9 | 0.7 | 1.0 |
| Fold change | 3.9 | 3.6 | 2.7 | 28.9 | −1.6 | 6.0 | 2.9 | −16.7 | 2.3 | |||
| CCL17 (TARC) | ||||||||||||
| Mean | 0.6 | 1.4 | 0.8 | 0.5 | 0.2 | 13.7*** | 3.8*** | 0.3 | 0.0 | 1.3*** | 0.7*** | 0.4*** |
| (SE) | (0.3) | (0.6) | (0.2) | (0.2) | (0.1) | (6.7) | (1.4) | (0.1) | (0.0) | (0.6) | (0.5) | (0.3) |
| Median | 0.4 | 1.2 | 0.8 | 0.3 | 0.2 | 10.2 | 3.0 | 0.2 | 0.0 | 0.8 | 0.3 | 0.0 |
| Fold change | 2.9††† | 1.2 | −0.6 | 94.3 | 26.7 | 2.0 | 49.5† | 19.8 | 13.5 | |||
| IL-13 | ||||||||||||
| Mean | 0.5 | 3.6** | 1.0 | 0.7 | 2.0 | 62.7*** | 11.1*** | 4.0 | 1.6 | 26.2*** | 13.9*** | 3.6** |
| (SE) | (0.1) | (0.8) | (0.3) | (0.2) | (0.4) | (13.0) | (1.8) | (0.8) | (0.6) | (8.8) | (6.1) | (1.0) |
| Median | 0.6 | 4.3 | 1.2 | 0.6 | 1.8 | 80.3 | 10.6 | 4.8 | 0.8 | 19.8 | 10.0 | 2.8 |
| Fold change | 7.7 | 1.5 | 0.8 | 31.6 | 6.6 | 1.8 | 34.6 | 8.1 | 2.8 | |||
| IL-5 | ||||||||||||
| Mean | 0.6 | 0.8 | 0.4 | 0.6 | 2.9 | 8.8** | 2.8 | 2.1 | 3.9 | 3.6 | 1.9 | 3.2 |
| (SE) | (0.1) | (0.2) | (0.1) | (0.2) | (0.1) | (3.8) | (0.6) | (0.2) | (1.7) | (0.5) | (0.5) | (0.3) |
| Median | 0.5 | 0.7 | 0.3 | 0.7 | 3.0 | 5.2 | 2.8 | 1.9 | 2.3 | 3.5 | 2.0 | 3.2 |
| Fold change | 0.8 | −1.3 | 0.4 | 3.0 | −0.1 | −1.5 | 0.6† | −1.7 | 0.6 | |||
| TNF-α | ||||||||||||
| Mean | 12.2 | 28.0* | 26.0* | 11.6 | 16.8 | 22.4 | 18.0 | 13.0 | 8.2 | 16.4** | 13.7 | 9.0 |
| (SE) | (4.0) | (9.8) | (5.1) | (1.8) | (4.5) | (4.6) | (1.9) | (1.7) | (1.6) | (1.8) | (5.2) | (1.8) |
| Median | 8.8 | 27.1 | 21.1 | 10.1 | 13.5 | 20.3 | 15.7 | 13.5 | 10.6 | 17.1 | 8.3 | 8.2 |
| Fold change | 2.4 | 2.3 | 0.3 | 1.2 | 0.8 | −0.4 | 2.4 | 0.7 | 0.4 | |||
| IL-10 | ||||||||||||
| Mean | 0.6 | 1.1 | 1.2 | 0.8 | 11.9 | 17.4 | 11.6 | 18.9 | 11.7 | 17.5 | 10.6 | 16.0 |
| (SE) | (0.1) | (0.2) | (0.3) | (0.1) | (1.2) | (2.3) | (2.5) | (3.1) | (3.0) | (3.1) | (2.3) | (2.6) |
| Median | 0.7 | 1.2 | 1.3 | 0.7 | 11.8 | 20.2 | 10.8 | 20.2 | 7.7 | 19.9 | 11.1 | 16.9 |
| Fold change | 1.9 | 1.9 | 0.7 | 1.5 | −0.4 | 1.2 | 1.1 | −0.3 | 1.6 | |||
***
P < 0.001
**
P < 0.01
*
P < 0.05, relative to baseline mRNA expression.
†††
P < 0.001
†† P < 0.01
†
†P < 0.05, decreased fold change compared to that seen in time-matched, anti-IgE injected skin in untreated dogs.