Figure 5.

IntSIIN is endocytosed and processed in endosomes. DC were incubated for 45 min with inhibitors (a) chloroquine/NH₄CL (200, 20 μm), and (b) monensin (1000, 100 μm) followed by incubation with IntSIIN at 1 μg/ml and added to B3Z T cells for 24 hr. DC were pulsed with SIINFEKL in the presence of the biochemical inhibitor at the maximum dose and added to B3Z T cells in parallel experiments to ensure no inhibitory effects on the presentation of SIINFEKL CTL epitope. LacZ activity in B3Z T cells was assayed by total culture lysates with LacZ substrate CPRG. The absorbance (560 nm) of culture wells was read after 4 hr incubation at 37°.