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. 2006 Mar;117(3):329–339. doi: 10.1111/j.1365-2567.2005.02304.x

Figure 6.

Figure 6

Proteolysis and peptide loading of IntSIIN. DC were incubated for 45 min with inhibitors, (a) lactacystin (10, 1 μm), (b) brefeldin (10, 1 μg/ml), (c) protease inhibitor (1/100, 1/10 dilution), (d) furin inhibitor (10, 1 μm) and (e) bestatin (10, 1 μm) followed by incubation with IntSIIN at 1 μg/ml and added to B3Z T-cell hybridoma for 24 hr. DC were pulsed with SIINFEKL in the presence of the biochemical inhibitor at the maximum dose and added to B3Z T cells in parallel experiments to ensure no inhibitory effects on the presentation of SIINFEKL CTL epitope. LacZ activity in B3Z T cells was assayed by total culture lysates with LacZ substrate CPRG. The absorbance (560 nm) of culture wells was read after 4 hr incubation at 37°.