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. 2006 Mar;117(3):409–418. doi: 10.1111/j.1365-2567.2005.02315.x

Figure 2.

Figure 2

A representative Southern blot (a) and PCR analysis (b) of the screening of transgenic mice.(a) Southern blot analysis of the genomic DNA isolated from mouse tails of the first generation mice after injection of the construct. On this blot four mice carry the transgene. (b) PCR analysis was performed on the fifth generation of transgenic mice. A PCR product of 800 bp because of the appearance of the p40 transgene in combination with the SP-C promoter was detected in all five mice tested. M = marker.