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. 2006 Mar;117(3):396–401. doi: 10.1111/j.1365-2567.2005.02313.x

Figure 2.

Figure 2

Coimmunoprecipitation of STAT5a and STAT5b with CTLA-4. Two micrograms HA-tagged STAT5a in pcDNA3 (a) or STAT5b in pcDNA3 (b)were transfected in HEK293T cells together with 2 μg CTLA-4-pcDNA3 or with the pcDNA3 empty vector, as indicated (+ or −). Between 24 and 36 hr after transfection, cells where harvested and lysed. Lysates were immunoprecipitated with anti-CTLA-4 mAbs, the immunoprecipitate was subjected to SDS–PAGE, blotted onto PVDF and immunostained with anti-HA mAbs (a) or anti-STAT5b polyclonal antibodies (b) (upper panel). Total lysates were separated by SDS–PAGE, blotted onto PVDF immunostained with anti-HA mAbs (a) or anti-STAT5b polyclonal antibodies (b) showing comparable amount of STATS5a/b in each lane.