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. 2006 Mar;117(3):396–401. doi: 10.1111/j.1365-2567.2005.02313.x

Figure 4.

Figure 4

CTLA-4 expression in Jurkat cells and its effect on a STAT5-responsive reporter. (a)Wild Jurkat E6.1 and CTLA-4-transfected Jurkat cells were incubated without or with doxycyclin. Following immunostaining with PE-conjugated CTLA-4 mAb, surface levels of CTLA-4 were analysed by flow cytometry. Dot plots of reactivity with PE-conjugated anti-CTLA-4 (y-axis) are shown. (b)Jurkat E6.1 or Jurkat cells stably transfected to express CTLA-4 were transfected with a GAS-luciferase reporter plasmid. Each transfection mixture was split into two different wells, one with and one without doxycyclin. 24 hr after transfection, samples were left unstimulated (– IL-2) or were stimulated with IL-2 (200 U/ml) (+ IL-2). The results are representative of four independent transfections.