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. 2006 Apr;117(4):443–451. doi: 10.1111/j.1365-2567.2006.02335.x

Figure 2.

Figure 2

Release of exogenous solute into the cytosol of a murine bone-marrow-derived DC. DC were cultured at 37°C in the presence of 1 mg/ml fluorescein isothiocyanate (FITC)-dextran (40 000 MW), then washed and chased in the presence of Trypan Blue that will quench FITC fluorescence if it is accessible from outside the cell. FITC fluorescence is not quenched, and so is in an intact living cell.