Figure 1.

CD40-mediated enhancement of surface molecule expressions and IL-6 and IL-12 production by immature and mature DCs. Unstimulated or LPS-treated BC1 cells were considered as immature (iDC) or mature DCs (mDC), respectively. (a) Cell surface expressions of CD40, CD86, and MHC class II (I-A^d). iDC and mDC were analysed by flow cytometry. (b) Cytokine production by iDCs and mDCs. The iDC and mDC were treated with anti-CD40 mAb (α-CD40) or isotype-matched control-IgM (isotype) for 24 hr. The amount of IL-6 and IL-12 in the culture supernatants was measured by ELISA. Each column represents the mean ± SE of triplicate wells. Data are representative of at least three independent experiments.