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. 2006 May;118(1):66–77. doi: 10.1111/j.1365-2567.2006.02342.x

Figure 8.

Figure 8

IL-12 derived from CD40-activated memory B cells induce T cells to produce IFN-γ. CD3+ CD4+ T cells were stimulated with PMA (10 ng/ml) and 5 µl of CD28/CD49d costimulatory antibodies with or without supernatants from naïve or memory B cells cultured for 3 days on dual CD32/CD40L-transfected L cells. After 3 days, supernatants were harvested and analysed for IFN- production by ELISA. Where indicated, neutralizing anti-IL-12 mAb (20 µg/ml) was added. Data are the mean ± SD for three separate experiments.