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Progressive contraction of HIV-specific CD8+ clonotype 18A following HAART in subject H0018 (I) Amplified TCR sequences were blotted onto a filter, which was first probed using the labelled clonotypic probe. (II) By stripping the filter and reprobing with a conserved constant region-specific probe that detects all amplified TCRs, we calculated the relative abundance of clonotype sequence as a proportion of all TCR sequences of the Vβ13.1 family.
