Table 2.
In subjects H0018 and H0009, before and after antiretroviral therapy multiple independent CD8+ formal CTL clones or clonal microcultures specific for a defined HIV peptide have identical TCR β-chain nucleotide sequences
| Donor | Peptide | Date | Clone or culture code | V | CDR3† | J | C | Clono-type | No. of identical clones or cultures | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H0018 | gag 703.3 | 06/96 | Clone 4.6 | Vβ5.2 | C | A | S | T | S | G | W | G | S | Y | E | Q | Y | Jβ2.7 | Cβ2 | 1/1 | |
| 01/98 | Clone 2 | Vβ13.1 | C | A | S | N | L | A | P | Y | E | Q | Y | Jβ2.7 | Cβ2 | 18A | 3/3 | ||||
| 04/98 | Culture 3 | Vβ13.1 | C | A | S | N | L | A | P | Y | E | Q | Y | Jβ2.7 | Cβ2 | ” | 2/2 | ||||
| 07/00 | Culture 1 | Vβ13.1 | C | A | S | N | L | A | P | Y | E | Q | Y | Jβ2.7 | Cβ2 | ” | 2/2 | ||||
| H0009 | env 740.19 | 09/96 | Clone 3.2 | Vβ13.6 | C | A | S | S | Y | T | L | D | N | Q | P | Q | H | Jβ1.5 | Cβ1 | 9A | 1/1 |
| 04/97 | Culture 1 | Vβ13.6 | C | A | S | S | Y | T | L | D | N | Q | P | Q | H | Jβ1.5 | Cβ1 | ” | 5/5 | ||
| 06/01 | Culture 1 | Vβ13.6 | C | A | S | S | Y | T | L | D | N | Q | P | Q | H | Jβ1.5 | Cβ1 | ” | 1/1 | ||
| 08/01 | Culture 1 | Vβ13.6 | C | A | S | S | Y | T | L | D | N | Q | P | Q | H | Jβ1.5 | Cβ1 | ” | 2/2 | ||
Bold type indicates cultures derived before starting antiretroviral therapy.
†
See Table 1 footnote.