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. 2006 May;118(1):101–111. doi: 10.1111/j.1365-2567.2006.02346.x

Figure 5.

Figure 5

Natural killer T (NKT) cells contributed to the induction of regulatory T cells producing interleukin (IL)-10 and transforming growth factor (TGF)-β1 in Peyer's patches (PPs). Spleen cells (2 × 106) from DO11·10 mice were adoptively transferred into BALB/c or BALB/c CD1d–/– mice, which were then fed ovalbumin (OVA) or phosphate-buffered saline (PBS). Immune cells were taken from the PPs and spleens of BALB/c and BALB/c CD1d–/– mice fed 250 mg (OVA-250) or 1 mg OVA (OVA-1) or PBS. (a) The production of IL-10, TGF-β, interferon (IFN)-γ and IL-4 was measured using enzyme-linked immunosorbent assay (ELISA) after stimulating immune cells (2 × 106 cells/ml) with OVA (100 μg/ml) from BALB/c mice (white bars) or BALB/c CD1d–/– mice (black bars). (b) The intracellular expression of TGF-β and IL-10 was evaluated in gated CD25+ cells by flow cytometry after stimulating immune cells with OVA. These results are taken from a representative experiment of five repeated experiments. SP, spleen.