Figure 1.

Dose–response effects of interleukin (IL)-10 and CTLA4-Ig in the DC-MLR. IL-10 (solid line) and CTLA4-Ig (dashed line) were titrated with 10-fold dilutions in the DC-MLR. Nylon wool T cells were used as the responder population at a stimulator:responder (S:R) ratio of 1 : 100. All samples were run in triplicate and proliferation was measured by [³H] thymidine incorporation, with results presented as counts per minute (c.p.m.) ± standard deviation (SD). The maturation status of the DCs was demonstrated by flow cytometric analysis by direct staining with anti-CD83-phycoerythrin (PE) (shaded) and with the negative control, isotype-matched PE-conjugated monoclonal antibody (unshaded). iDC, immature dendritic cell; mDC, mature dendritic cell.