Figure 6.
Bifidobacterium infantis attenuates flagellin-induced interleukin (IL)-8 secretion. Confluent HT-29 cells were pretreated for 2 hr with B. infantis or Lactobacillus salivarius at doses of 1 × 106 or 1 × 107 colony-forming units (CFU)/ml prior to stimulation with 0·5 µg/ml flagellin for 6 hr. Pretreatment with 1 × 107 CFU/ml B. infantis, but not L. salivarius, significantly inhibited flagellin-induced IL-8 secretion (*P < 0·05 relative to flagellin-treated HT-29 monolayers). The data are expressed as pg/ml IL-8 and represent the mean ± standard error of three independent experiments.