Figure 1.

The dual altered peptide ligand (APL) up-regulates expression of Fas and FasL on p195–212-specific T cells. Cells (10⁶ per ml) of the line were stimulated with p195–212 (5 µg per ml) with or without the dual or reversed dual APL (500 µg per ml) for 24 hr. Cells were double-stained for CD45Rb and Fas or FasL and analysed by fluorescence activated cell sorter (FACS). The results presented are after reduction of the background staining obtained with the matched isotype controls. Numbers in the upper levels correspond to CD45Rb^high cell population and those below correspond to CD45Rb^1ow cells. Shown is one of two experiments performed.