Figure 6.

The dual altered peptide ligand (APL) up-regulates Fas and FasL expression in mice immunized with Torpedo acetylcholine receptor (TAChR). C57BL/6 mice were either immunized with TAChR [10 µg per mouse in complete Freund's adjuvant (CFA)] and concomitantly administered subcutaneously (s.c.) with the dual APL/reversed dual APL [200 µg per mouse in phosphate-buffered saline (PBS)] or immunized with TAChR alone. Ten days after immunization, popliteal lymph node (LN) cells were harvested. LN cells were stained for CD4, CD25, and Fas or FasL and analysed by fluorescence activated cell sorter (FACS). The results presented are after reduction of the background staining obtained with the matched isotype controls. Shown is one of three experiments performed.