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. 2006 Jul;118(3):333–342. doi: 10.1111/j.1365-2567.2006.02374.x

Figure 4.

Figure 4

Western blot analysis of CD46 (a) & CD55 (b & c) isoforms. (a) PBMC (lane 1), RBC (lane 2) and AR-spermatozoa (lane 3) cell lysates separated by SDS-PAGE and reacted with anti-CD46 pAb. (b) PBMC (lane 1), blank (lane 2) and AR-spermatozoa (lane 3) cell lysates. (c) Deglycosylation analysis of HeLa cell (lanes 1–4) and AR-spermatozoa (lanes 5–8) cell lysates, either untreated (lanes 1 & 5) or treated with N-glycosidase (N: lanes 2 & 6), O-glycosidase and neuramidase (O: lanes 3 & 7) or N-glycosidase, O-glycosidase and neuramidase (N,O: lanes 4 & 8), separated by SDS-PAGE and reacted with anti-CD55 mAb.