Figure 3.

Cytokine responses in T-bet^−/− (Tb^−/−) and wild type (wt) littermates. Spleen cells from PLP(_180−199)-immunized mice were harvested at two different time points of disease course [days 15 (a and c) and 30 (b and d)] and cells re-stimulated ex vivo with antigen PLP(_180−199) (1–10 µg/ml) peptide for cytokine (IFN-γ and IL-10) responses. The supernatants were collected at 48 hr (for IFN-γ) and 96 hr (for IL-10) for cytokine measurements. The IFN-γ and IL-10 secretion at day 15 (a and c) and day 30 (b and d) are shown here. The data represents results (mean ± SD) of three independent experiments. ***P < 0·0001 compared to T-bet^−/− at different antigen concentrations.