Figure 4.
CD4 help does not result in the formation of memory CD8 T cells. Naive HA-specific CD8 T cells were adoptively transferred into C3-HAhi mice alone (Control) or with HA-specific CD4 T cells derived from 6.5 TCR-HA transgenic mice (+ 6.5). Four days later, lymphocytes from peripheral lymph nodes were harvested for the percentage and MFI of IFN-γ-secreting cells among clonotypic HA-specific CD8 T cells (a). Four, 7, 14, or 21 days later, the percentages of clonotypic HA-specific CD8 T cells in total lymphocytes were analysed (b).