Figure 5.

Antigen duration in CD8 T-cell memory versus tolerance. (a) A schematic view of the experiments outlined below. (b) Naive HA-specific CD8 T cells were adoptively transferred into B10.D2 mice infected with rVV-HA (rVV-HA) or C3-HA^hi mice on day 0. At days 0 (d0), 7 (d7), 10 (d10) or 14 (d14), CFSE-labelled naive HA-specific T cells were transferred into these mice and in vivo divisions of CFSE-labelled cells harvested from the spleen were analysed 4 days later. Events were gated on CD8⁺ Thy-1.1⁺ T cells. Representative data of three independent experiments are shown.