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Tonsil sections stained with antibodies against (a) CD20 (diluted 1 : 1500), (b) CD3 (diluted 1 : 100), (c) Toll-like receptor (TLR)1 (diluted 1 : 50), (d) TLR2 (diluted 1 : 50), (e) TLR7 (diluted 1 : 50) and (f) TLR9 (diluted 1 : 50), were localized using 3,3′-diaminobenzidine (DAB), which stains tissues brown, and analysed by microscopy (magnification ×40–100).
