Table 3.

Primer sequences used for the amplification of cytokine, inducible nitric oxide synthase (iNOS) and housekeeping gene transcripts

Primer	Sequence (5′−3′)	Acc. no., position
β-Actin S	CGT TGC TAT CCA GGC TGT GC	M10277, 2064–83
β-Actin AS	GTA GTT TCG TGG ATG CCA CA	M10277, 2593–2574
IL-2 S	AGA TGG AGC AAT TAC TGC TGG	U28141, 139–159
IL-2 AS	ATT CTG TGG CCT TCT TGG GCG TGT	U28141, 258–235
IL-10 S	CCT GGG TTG CCA AGC CCT GTC	U338433, 235–255
IL-10 AS	ATG CGC TCT TCA CCT GCT CC	U338433, 446–427
IL-12 (p40)S	CAC CTG CCA TAC CCC TGA AG	U49100, 144–163
IL-12 (p40)AS	TGA CCC TCT CTG CTG AAA GT	U49100, 541–522
TGF-β S	TTC CTG CTC CTC ATG GCC AC	L34956, 826–854
TGF-β AS	GCA GGA GCG CAC GAT CAT GT	L34956, 1218–1199
TNF-α S	CCA AGT GAC AAG CCA GTA GC	Z70046, 32–51
TNF-α AS	TCT TGA TGG CAG AGA GTA GG	Z70046, 305–286
IFN-γ S	GCA AGT AAT CCA GAT GTA TCG	S41201, 67–87
IFN-γ AS	TTA TCG CCT TGC GCT GGA CC	S41201, 349–330
iNOS S	AGA CAC ACT TCA CCA CAA GG	AF077821, 369–388
iNOS AS	TGC TTG GTG GCG AAG ATG AGC	AF077821, 653–633

Acc. no. = GenBank Accession no.; S = sense primer, AS = antisense primer.