Skip to main content
. 2006 Sep 1;119(1):8–17. doi: 10.1111/j.1365-2567.2006.02383.x

Figure 3.

Figure 3

AdV-SOCS3 does not regulate TNF-α mRNA induced by LPS or LPS + IL-10. Total RNA was isolated from uninfected, AdV-GFP- and AdV-SOCS3-infected monocytes following treatment with 500 ng/ml LPS (open bars) or LPS in the presence of 10 ng/ml IL-10 (solid bars) for 60 min. TNF-α mRNA levels were measured using real time PCR and normalized to the housekeeping gene UBE2D2 (Ubiquitin conjugating enzyme E2D2). Values are the mean ± SEM of three replicates. Statistical significance was calculated using a one-way anova (*P < 0·05). The data are representative of two experiments from individual donors. TNF-α mRNA at time 0 was negligible on this scale for uninfected cells and AdV-GFP and AdV-SOCS3-infected monocytes (data not shown).