Table 2.
Cytokine responses and levels of serological markers in human immunodeficiency virus (HIV) patients (n = 19)
| IFN-γ ELISPOT1 | IFN-γ mRNA2 | IL-4 mRNA | IL-4δ2 mRNA | IL-2 mRNA | IL-5 mRNA | |
|---|---|---|---|---|---|---|
| Unstimulated | 7 | 12·5 | 0·10 | 0·001 | 0·39 | 0·001 |
| (1–47)3 | (2·3–20) | (0·002–3·8) | (0·001–0·003) | (0·13–0·79) | 0·001 | |
| CMV stimulated | 10 | 18·5 | 0·14 | 0·001 | 3·2 | 0·001 |
| (0–206) | (10–33) | (0·06–0·47) | (0·001–0·008) | (0·13–18) | (0·001–0·09) | |
| Anti-CD3 stimulated | 481 | 48·8 | 0·47 | 0·001 | 8·4 | 0·01 |
| (120–989) | (19–110) | (0·08–2·6) | (0·001–0·11) | (0·13–32) | (0·001–0·07) | |
| PHA stimulated | >400 | 63·1 | 1·87 | 0·06 | 61 | 0·04 |
| (15–171) | (0·16–8·7) | (0·008–0·18) | (0·13–274) | (0·002–0·14) |
| LAG-3 pg/ml | CD30 U/ml | CD26 U | IgE kU/l |
|---|---|---|---|
| <22 | 19·7 | 164 | 78 |
| (<22–360) | (6·0–64·9) | (76–276) | (0·5–1028) |
1
Spots per 2 × 105 peripheral blood mononuclear cells (PBMC).
2
Ratio to β-actin mRNA.
3
Median (range).
CMV, cytomegalovirus; ELISPOT, enzyme-linked immunosorbent spot-forming cell assay; IFN-γ, interferon-γ; IL, interleukin; LAG-3, lymphocyte activation gene-3; PHA, phytohaemagglutinin.