Table 4.
Levels of T1 and T2 cytokine mRNA correlated directly with CD30 and inversely with immunoglobulin E (IgE) levels
| Cytokine response | Serological marker | Correlation coefficient | P-value |
|---|---|---|---|
| Unstimulated IFN-γ mRNA | LAG-3 | 0·44 | 0·06 |
| IFN-γ ELISPOT to anti-CD3 | CD30 | 0·41 | 0·10 |
| IL-5 mRNA to anti-CD3 | CD30 | 0·40 | 0·10 |
| IL-5 mRNA to PHA | CD30 | 0·47 | 0·05 |
| IL-4δ2 mRNA to PHA | CD30 | 0·51 | 0·03 |
| IFN-γ ELISPOT to anti-CD3 | IgE | −0·46 | 0·05 |
| IFN-γ mRNA to anti-CD3 | IgE | −0·51 | 0·03 |
| IL-5 mRNA to anti-CD3 | IgE | −0·52 | 0·02 |
| IL-4 mRNA to anti-CD3 | IgE | −0·45 | 0·05 |
| IL-4δ2 mRNA to anti-CD3 | IgE | −0·62 | 0·004 |
| IL-5 mRNA to PHA | IgE | −0·38 | 0·10 |
| IL-4δ2 mRNA to PHA | IgE | −0·41 | 0·08 |
Cytokine mRNA levels are expressed as a ratio to β-actin mRNA. Values for unstimulated cells were subtracted from the values for stimulated cells. Only comparisons yielding P ≤ 0·10 are shown.
ELISPOT, enzyme-linked immunosorbent spot-forming cell assay; IFN-γ, interferon-γ; IL, interleukin; IL-4δ2, inhibitory splice variant of IL-4; LAG-3, lymphocyte activation gene-3; PHA, phytohaemagglutinin.