Figure 1.

Epicutaneous immunization enhances T-cell responses to subsequent immunization with CFA. DTH responses (a) were measured 24 hr after single peanut protein challenge in the footpad and are expressed as mean increment of footpad swelling + 1 SEM (n = 7). T cells taken from PLN draining the peanut/CFA immunization site 7 weeks after the initial epicutaneous immunization were cultured for 90 hr with peanut protein or control antigen (▪) and proliferation was determined by [³H]thymidine incorporation (b) Results are expressed as mean c.p.m. ± 1 SEM. Background proliferation when no antigen was present has been subtracted. All groups responded with similar c.p.m. when stimulated with Con A. Experimental groups and order of procedures are listed in the inset table.