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. 2006 Sep 1;119(1):36–42. doi: 10.1111/j.1365-2567.2006.02402.x

Figure 3.

Figure 3

IFN-α expression in PDCs in response to SeV with or without PGE2 by RT-PCR and intracellular staining. PDCs were cultured for 8 hr with SeV plus vehicle or SeV plus PGE2 (10−6 m). (a) IFN-α2 mRNA expression in PDCs was analysed by RT-PCR. (b) IFN-α staining in PDCs was performed intracellularly by flow cytometry. Percentages of IFN-α-producing PDCs are indicated. Viable PDCs were analysed by exclusion of dead cells on the basis of side- and forward-scatter characteristics. Data represent one of three experiments.