Figure 5.

CP-Siase and OSGE pretreatment increase CD3/CD28- and IL-2-induced granzyme B expression in CD8 T cells. (a) T cells from young (Y) and old (O) mice were either left untreated or were treated with CP-Siase or OSGE, and then stimulated with cross-linked anti-CD3 and anti-CD28 for 6 hr (upper panels), or incubated with 100 IU of recombinant IL-2 for 72 hr (lower panels). For each set of samples, gated CD8 T cells were examined for granzyme B expression at the end of the incubation period. The graphs illustrate one experiment of the four used in the statistical analysis. The far left trace in each panel is the isotype control. (b) Mean fluorescence intensities (MFI) of granzyme B expression for all experiments conducted. The symbols * and † indicate statistically significant difference with untreated young or old, respectively. **Denotes statistically significant difference between young and old.