Figure 1.

Differential expression of CXCL13 in wild type and Pactolus-deficient animals. (a) Four BALB/c and four Pactolus-deficient mice (bred upon BALB/c background for seven generations) were induced with a peritoneal injection of thioglycollate, cells were isolated from the cavity 16 hr later. Quantitative RT–PCR was done on each sample independently comparing CXCL13 transcripts per 1 × 10³β-actin transcripts. *t = 0·06. (b) Eight 129/sv and four BALB/c mice were induced with a peritoneal injection of thioglycollate and cells were isolated from the peritoneal cavity 16 hr later. Quantitative RT–PCR was done comparing CXCL13 transcripts per 1 × 10³β-actin transcripts on each individual sample. (c) Percentage of GR-1 positive cells (granulocytes) in a peritoneal lavage 16 hr after thioglycollate treatment between the BALB/c (five mice examined and averaged) and Pactolus-deficient animals (five animals examined and averaged). (d) Cells were taken from a naïve peritoneal lavage from five 129/sv, five BALB/c and four Pactolus-deficient animals. RNA was obtained from the individual cell populations and analysed for CXCL13 transcripts per 1 × 10³β-actin transcripts. Analysis of single experiments is shown but each were done multiple times and generated the same data.