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. 2006 Oct;119(2):212–223. doi: 10.1111/j.1365-2567.2006.02426.x

Figure 4.

Figure 4

Expression of CXCL13 in in vitro cultures. (a) Total peritoneal washes from naïve animals were incubated alone (WASH) or with bone marrow isolated neutrophils from wild type (BALB/c PMN WASH) or from Pactolus-deficient animals (PacKO PMN WASH), for 16 hr in tissue culture. Equivalent samples of the isolated neutrophils (BALB/c PMN or PacKO PMN) were also analysed in parallel. Relative levels of CXCL13 transcripts were obtained from identical experiments and normalized to a 100% value from the WASH sample. (b) Total peritoneal washes from wild type (BALB/c Macrophage) and Pactolus-deficient (PacKO Macrophage) animals 96 hr after thioglycollate treatment were incubated either alone (Blank) or with neutrophils isolated from the bone marrow of Pactolus-deficient (PacKO PMN) or wild type (BALB/c PMN) animals. The analysis of a single experiment is shown but is indicative of a number of independent analyses that provided the same result.