Table 1.
Primary antibodies used in the study
| Recognized cells | Antibody specificity | Antibodies | Source of antibody |
|---|---|---|---|
| Epithelial cell | pan cytokeratin (cross-reactive with chicken) | clone Lu-5 (mouse IgG1) | BMA, Biomedicals AG Augst, Switzerland |
| Chicken epithelial cells | chicken E-cadherin | 7D6 (mouse IgG1) | DSHB, Iowa, IA |
| Haematopoietic cell | chicken CD45 antigen | clone HIS-C7 (mouse IgG2a) | gift from Dr H. KurzAlbert-Ludwigs University Freiburg, Germany |
| pan T cell | CD3 antigen (cross-reactive with chicken) | polyclonal anti-human pan T cell(rabbit IgG) | Dako A/S, Glostrup, Denmark |
| Th cell | chicken CD4 | CT-4 (mouse IgG1) | Southern Biotechnology Associates, Birmingham, AL |
| Tc cell | chicken CD8 | CT-8 (mouse IgG1) | Southern Biotechnology |
| T cell αβ | chicken (Vβ1) TCR-αβ | TCR-2 (mouse IgG1) | gift from Dr Thomas Göbel,Ludwig Maximilian University, Munich, Germany |
| T cell αβ | chicken (Vβ2) TCR-αβ | TCR-3 (mouse IgG1) | gift from Dr Thomas Göbel, Ludwig Maximilian University,Munich |
| T cell γδ | chicken TCR-γδ | TCR-1-FITC (mouse IgG1) | Southern Biotechnology |
| B-cell and macrophage subpopulation | Bu-1b antigen | clone 5-11G2 (mouse IgG1) | Southern Biotechnology |
| Different cell types | vimentin intermediate filament | VIM 3B4 (mouse IgG2a) | Progen, Biotechnik GmbH, Heidelberg |
| Different cell types | vimentin intermediate filament | AMF17b (mouse IgG1) | DSHB, Iowa, IA |
| chicken IgG | clone CG-106 (mouse IgG1) | Sigma-Aldrich Kft, Hungary | |
| chicken IgM | clone M-1 (mouse IgG2b) | Southern Biotechnology | |
| Follicular dendritic cells | Unknown | CVI-ChNL-74.3 (mouse IgG1) | CEDI-Diagnostics, Lelystad, the Netherlands |
| Chicken reticularellipsoidal cells | 45 kDa protein | CVI-ChNL-68.2 (mouse IgG1) | CEDI-Diagnostics |
| Chicken macrophages | Unknown | CVI-ChNL-74.2 (mouse IgG1) | CEDI-Diagnostics |
| Chicken pan-mononuclear phagocyte | Unknown | CVI-ChNL-68.1 (mouse IgG1) | CEDI-Diagnostics |
| Chicken mononuclear phagocyte | Unknown | KUL01(mouse IgG1) | Gift from Dr B.M. Goddeeris, KU, Leuven, Belgium |
| chicken monocyte/macrophage/ thrombocyte | 145 kDa protein | K1 (mouse IgG1) | Gift from H. Lillehoj, FDA Washington, DC |
| B-cell, activated T-cell and dendritic cells | MHC II | clone 2D5 (mouse IgG2b) | DSHB, Iowa, IA |
| Chicken granulocytes | Unknown | GRL1 (mouse IgG3) | DSHB, Iowa, IA |
| Chicken granulocytes | Unknown | GRL2 (mouse IgG1) | DSHB, Iowa, IA |
| Lysosomal membrane | LEP 100 | LEP 100 IgG (mouse IgG1) | DSHB, Iowa, IA |
| Different cell types | chicken integrin α5 subunit | D71E2 (mouse IgG) | DSHB, Iowa, IA |
| Different cell types | chicken integrin α6 subunit | P2C62C4 (mouse IgG1) | DSHB, Iowa, IA |
| Different cell types | chicken integrin α7 subunit | H1B4 (mouse, unknown isotype) | DSHB, Iowa, IA |
| Different cell types | chicken integrin β1 subunit | CSAT (mouse IgG2b) | DSHB, Iowa, IA |
| Anti-caveolin-1 | Caveolin-1 | Polyclonal rabbit IgG | Gift from Dr A. L. Kiss, SU, Budapest, Hungary |
| Chicken skin melanocytes | unknown | MelEM (mouse IgG1) | DSHB, Iowa, IA |