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. 2006 Dec 14;26(1):79–89. doi: 10.1038/sj.emboj.7601448

Figure 7.

Figure 7

TRF1/BRF complex binding to proximal promoter of snoRNA:644 gene is essential for the gene activity. (A) DNase I footprinting assay utilizing a snoRNA:644 template and recombinant TRF1/BRF complex. The footprint region spanning approximately 25 bp is indicated by a black box and sequences on the forward strand of the gene. (B) Schematic representation of the snoRNA:644 templates used for the promoter deletion experiments. pCR4 vector backbone is omitted from the diagram. The numbers represent the position of the promoter fragment relative to the transcription start site determined by primer extension analysis. (C) In vitro transcription of the snoRNA:644 templates described above. The snoRNA:644 promoter retains activity even when the majority of gene external sequence is removed. (D) Schematic representation of the snoRNA:314 templates used for the promoter deletion experiments. pCR4 vector backbone is omitted from the diagram. The numbers represent the position of the promoter fragment relative to the transcription start site determined by primer extension analysis. (E) In vitro transcription of the snoRNA:314 templates described in (D).