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The distribution of HUP1GFP is impaired in mutants deficient in mitochondrial respiration. Surface scans of WT cells (A), atp1Δ and cox7Δ mutants (B, C), all expressing HUP1GFP. For atp1Δ and cox7Δ, a considerably lowered membrane potential was detected by measurement of the uptake of 3H-TPP as compared to untreated and FCCP-treated WT cells (D). Bar: 2 μm.
