Figure 8.

Leakage of AIB from the cells with depolarized plasma membrane. Cells accumulating radioactive AIB (strain BY4742) were treated with 0.1% SDS (arrowhead) in the absence (•) or presence of 20 μM FCCP (▴). The efflux of accumulated radioactivity was much slower in the presence of FCCP. Considerably higher detergent concentration (0.5% SDS; ▪) was necessary to induce an effect comparable to that observed in FCCP-untreated cells. Note that the addition of FCCP at t=10 min (arrow) had no effect on the membrane permeability for AIB (◊).